Structure-Function Relationships of Cellular Retinoic Acid-binding Proteins
نویسندگان
چکیده
منابع مشابه
Cellular Retinol- and Retinoic Acid-binding Proteins of Bovine Retina
A three-step purification has been developed for the cellular retinoland cellular retinoic acid-binding proteins from extracts of bovine retina. The two binding proteins elute together in Step 1 (gel filtration) and separate in Step 2 (DEAE-cellulose chromatography), and each is purified to apparent homogeneity in the third step (calcium phosphate gel chromatography). The retinoland retinoic ac...
متن کاملRadioimmunochemical Determination of Cellular Retinoic Acid-binding Proteins in Cytosols
Radioimmunoassays have been developed for cellular retinol-binding protein and cellular retinoic acidbinding protein, postulated mediators of vitamin A action in nonvisual functions. These assays are considerably more sensitive for detection and quantitation of these proteins than previous methods. The higher sensitivity has permitted the detection of cellular retinoic acid-binding protein in a...
متن کاملPhotoaffinity labeling of retinoic acid-binding proteins.
Retinoid-binding proteins are essential mediators of vitamin A function in vertebrate organisms. They solubilize and stabilize retinoids, and they direct the intercellular and intracellular trafficking, transport, and metabolic function of vitamin A compounds in vision and in growth and development. Although many soluble retinoid-binding proteins and receptors have been purified and extensively...
متن کاملRelationships among retinoid structure, inhibition of growth, and cellular retinoic acid-binding protein in cultured S91 melanoma cells.
S91 melanoma cells, which are sensitive to retinoic acid and contain a cellular retinoic acid-binding protein (RABP), were used in an investigation of the possible correlation between the capacities of various retinoids to inhibit cell proliferation and to bind to the RABP. Each of 27 different retinoids was evaluated for growth-inhibitory activity by exposing S91 melanoma cells to various reti...
متن کاملRelationships among Retinoid Structure, Inhibition of Growth, and Cellular Retinoic Acid-binding Protein in Cultured S91 Melanoma Cells1
Retinoids.The structuresof the variousretinoidsusedinthis study are shown in Chart 1. The retinoids were the generous gifts of Dr. Beverly A. Pawson of Hoffman-La Roche Inc. (Nutley, N. J.) and Dr. Fritz F. Fnickel of BASF Aktiengesells chaft (Ludwigshafen, Federal Republic of Germany). All reti noids were stored under a nitrogen atmosphere in sealed containers at —70° and dissolved in ethyl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1997
ISSN: 0021-9258
DOI: 10.1074/jbc.272.3.1541